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Cannabis sativa and Humulus lupulus essential oils as novel control tools against the invasive mosquito Aedes albopictus and fresh water snail Physella acuta

机译:大麻和Hum草精油作为新型控制工具,可对抗入侵蚊子白纹伊蚊和淡水蜗牛Physella acuta

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摘要

Over the past several decades, there has been a resurgence of interest in industrial hemp (Cannabis sativa L., Cannabaceae) cultivation. Besides fibre, seeds and oil, hemp contains high quantity of essential oil (EO). Hop (Humulus lupulus L., Cannabaceae) is a high-climbing, perennial vine, largely utilized in the brewing industry to add flavour and bitterness to beer. While it is known that hop also contains α- and ß-acids, and terpenes that have been found to be toxic, anti-feedant, and repellent for insects and mites, little is known about the bioactivity against problematic species of the hemp EO. In this study, the chemical composition of the EOs from C. sativa and H. lupulus was evaluated by GC-MS, and their acute toxicity was assessed against the Asian tiger mosquito Aedes albopictus (Skuse) (Diptera Culicidae) and, the freshwater bladder snail Physella acuta (Draparnaud) (Mollusca Physidae), two problematic invasive species. Furthermore, we evaluated the toxicity of both EOs against a non-target insect, the mayfly Cloeon dipterum L. (Ephemeroptera Baetidae). Both EOs were toxic against the three tested species. The most effective EO was the C. sativa, able to kill 100% of P. acuta snails starting from 100 μL L-1. C. sativa LC50 were 301.560, 282.174 and, 35.370 μL L-1, while H. lupulus LC50 were 330.855, 219.787 and, 118.653 μL L-1 against A. albopictus, C. dipterum and P. acuta, respectively. Relative median potency analysis showed that the C. sativa EO was more toxic than H. lupulus against A. albopictus and P. acuta, while H. lupulus was more toxic than C. sativa EO against C. dipterum. The most susceptible species to the two EOs was P. acuta, while A. albopictus resulted the least susceptible one.
机译:在过去的几十年中,对工业用大麻(Cannabis sativa L.,Cannabaceae)种植的兴趣重新出现。除纤维,种子和油外,大麻还含有大量的精油(EO)。啤酒花(Humpus lupulus L.,Cannabaceae)是一种多年生的高攀藤本植物,主要​​用于酿造工业,以增加啤酒的风味和苦味。虽然已知酒花还含有α-酸和ß-酸,以及萜烯,它们被发现具有毒性,抗饲料性和对昆虫和螨虫的驱避性,​​但对大麻EO中有问题物种的生物活性知之甚少。在这项研究中,通过气相色谱-质谱法评估了苜蓿中华。和印度H的EOs的化学成分,并评估了它们对亚洲虎蚊白斑伊蚊(Skuse)(Diptera Culicidae)和淡水膀胱的急性毒性。蜗牛Physella acuta(Draparnaud)(Mollusca Physidae),两个有问题的入侵物种。此外,我们评估了两种EO对非靶标昆虫,即may蝇Cloeon dipterum L.(Ephemeroptera Baetidae)的毒性。两种EO对三种被测物种均具有毒性。最有效的EO是紫花苜蓿,能够杀死从100μLL-1开始的100%的P. acuta蜗牛。紫花苜蓿的LC50分别为301.560、282.174和35.370μLL-1,而l苜蓿的LC50分别为白斑拟南芥,二倍体梭菌和a。acuta的330.855、219.787和118.653μLL-1。相对中值效能分析表明,苜蓿中华绒螯蟹对白粉虱和角实衣藻的毒性要比棉铃虫更强,而苜蓿中华绒螯蟹的抗斜纹夜蛾的毒性要比普通苜蓿EO更高。对这两个EOs最敏感的物种是阿科毕赤酵母,而白色拟南芥导致的最不敏感。

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